Western blot analysis of extracts from HeLa cells using β-Actin (8H10D10) Mouse mAb as the primary antibody. Rabbit Anti-mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) (left) and Rabbit Anti-mouse IgG (HRP Conjugate) (right) secondary antibodies were used.
Western blot analysis of decreasing concentrations of total mouse IgG reduced and denatured in 1x SDS loading buffer with DTT using Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) (left) or Anti-Mouse IgG (HRP Conjugate) (right).
Product Name | Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) |
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Antibody Type | Secondary Antibodies |
Product description |
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Clonality | Monoclonal |
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Tested Applications | |
Species Reactivity | |
Concentration | 1 mg/ml |
Purification |
Tissue Specificity | Rabbit Anti-mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) recognizes native mouse IgG. It also recognizes the denatured and reduced mouse IgG light chains (about 25 kDa) on a western blot. |
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Western blot analysis of extracts from HeLa cells using β-Actin (8H10D10) Mouse mAb as the primary antibody. Rabbit Anti-mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) (left) and Rabbit Anti-mouse IgG (HRP Conjugate) (right) secondary antibodies were used.
Western blot analysis of decreasing concentrations of total mouse IgG reduced and denatured in 1x SDS loading buffer with DTT using Rabbit Anti-Mouse IgG (Light Chain Specific) (D3V2A) mAb (HRP Conjugate) (left) or Anti-Mouse IgG (HRP Conjugate) (right).
Storage Instructions |
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Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
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